Anxiolytic and Antioxydant Effects of Aqueous Extract of Hiptis spicigera Lam in Mice Exposed to Classical Paradigms and Chronic Immobilisation Test
Jean Pierre Omam Omam,
Andre Hamadou,
Samuel Mbouh,
Juliette Koube,
Dang Bouba Kadjou,
Mireille Delphine Ze Minkoulou,
Fleur Clarisse Moto Okomolo,
Elisabeth Ngo Bum
Issue:
Volume 6, Issue 4, December 2022
Pages:
50-61
Received:
9 October 2022
Accepted:
7 November 2022
Published:
8 December 2022
DOI:
10.11648/j.cnn.20220604.11
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Abstract: Anxiety is defined as an exaggerated feeling of apprehension, uncertainty and fear. The pharmacological treatments used for this disease show many side effects, which has limited their use by the patients. However, it is worth mentioning that in Africa medicinal plants are invaluable resources for the vast majority of rural populations, where more than 80% use them for their primary health care. This reality could justify the use of plants in African traditional medicine to treat neuropsychiatric diseases. The aim of this study is to assess the anxiolytic properties of Hyptis spicigera Lam leaves decoction on chronic immobilisation after treated by classical tests as EPM, Open Field and Hole Board. In order to assess the anxiolytic activity of the decoction on acute and chronic anxiety, the mice were divided by groups and treated with distilled water, Diazepam and different doses of the decoction (22.5mg/kg, 56.25mg/kg, 112.5mg/kg and 225mg/kg). Finally, some oxidative stress parameters such like catalase, sulfoxide dismutase, reduced glutathione, nitrite and malondialdehyde were measured. Concerning EPM test, the figure 1A and B showed a significant increase respectively of number of entries 6.8±1 and time spent in the open arms 94 ± 22.74 s from the negative control to 13.6 ± 2.3 and 210 ± 11 s in 225mg/kg dose of plant. Also, the figure 1C showed a significant decrease of the percentage of time spent in the closed arms from 68.7 to 30%. These parameters showed the reduction of the level of anxiety in these mice. The Open Field and Hole Board tests, by their parameters also showed that the Hyptis spicigera decoction would have anxiolytic properties. This could be justified may be by the presence of secondary metabolites such as saponins and flavonoids. The assay of oxidative stress markers showed that mice group which received the decoction and diazepam, had elevated catalase, sulfoxide dismutase, reduced glutathione and nitrites, but their malondialdehyde was low. The results of our study have shown that the decoction of Hyptis spicigera Lam would have anxiolytic and antioxydant properties.
Abstract: Anxiety is defined as an exaggerated feeling of apprehension, uncertainty and fear. The pharmacological treatments used for this disease show many side effects, which has limited their use by the patients. However, it is worth mentioning that in Africa medicinal plants are invaluable resources for the vast majority of rural populations, where more ...
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A Case of Sjogren’s Syndrome with Sensory Ganglionopathy
Maliha Hakim,
Mohammad Nur Uddin,
FM Monjur Hasan,
Mim Tanzila Mamun,
Alif Al Mamun,
Mashfiqul Hasan
Issue:
Volume 6, Issue 4, December 2022
Pages:
62-65
Received:
29 October 2022
Accepted:
5 December 2022
Published:
15 December 2022
DOI:
10.11648/j.cnn.20220604.12
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Abstract: Sensory ganglionopathy may associate with Sjögren’s syndrome (SS) and its clinical presentation is dominated by symptoms of damage to the type 1a large sensory fibers. Here we describe a case of SS with sensory ganglionopathy. A 27-year-old lady presented with imbalance and tingling-numbness of all limbs for 4 months. Imbalance aggravated during standing and walking, and was more marked in the dark. Tingling and numbness were asymmetrical, more marked in the upper limbs. She also complained of dry mouth and feeling of stickiness during swallowing for 9 months along with significant weight loss (about 25-kg over 6 months). Examination revealed hypotonia and areflexia, but muscle power was normal. There was an athetoid movement of the upper limbs, more marked after closing eyes. All modalities of sensation were impaired in all limbs. Romberg’s sign was positive and she had an unstable gait. Investigation revealed positive ANA, positive RNP, and positive SS-A. Shirmer’s test and saliva flow test was positive. Histopathological examination of labial mucosa revealed a Focus score of >1. Nerve conduction study (NCS) revealed absent sensory nerve action potentials (SNAP) both upper and lower limb nerves. Magnetic resonance imaging (MRI) of the spine revealed mild thickening of dorsal root ganglia and corresponding nerve roots. A diagnosis of primary Sjogren’s syndrome with ganglionopathy was made and the patient was started on corticosteroids and azathioprine. Neurologists should be aware of possible autoimmune origins of neuropathies and neuronopathies, as these are potentially treatable condition.
Abstract: Sensory ganglionopathy may associate with Sjögren’s syndrome (SS) and its clinical presentation is dominated by symptoms of damage to the type 1a large sensory fibers. Here we describe a case of SS with sensory ganglionopathy. A 27-year-old lady presented with imbalance and tingling-numbness of all limbs for 4 months. Imbalance aggravated during st...
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